Sylabus predmetu P15-0018-I - Methods and Techniques of Genetic Manipulations (FBFS - SS 2019/2020)

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University: Slovak University of Agriculture in Nitra
Faculty: Faculty of Biotechnology and Food Sciences
Course unit code: P15-0018-I
Course unit title: Methods and Techniques of Genetic Manipulations
Planned types, learning activities and teaching methods:
lecture2 hours weekly / 26 hours per semester of study (on-site method)
seminar2 hours weekly / 26 hours per semester of study (on-site method)

Credits: 6
Semester: Agrobiotechnology - master (required), 2. semester
Applied Biology - master (required), 2. semester
Applied Biology - master (required), 2. semester
Genetic Technologies in Agrobiology - master (elective), 2. semester
Level of study: 2.
Prerequisites for registration: none
Assesment methods :
During the semester there will be 2 written tests after 15 points, project presentation for 10 points and a final written exam for 60 points. To obtain the evaluation A it is necessary to obtain at least 93 points, for the evaluation B at least 86 points, for the evaluation C at least 79 points, for the evaluation D at least 72 points and for the evaluation E at least 64 points.
Learning outcomes of the course unit:
The student controls the basic principles of DNA cloning, nucleic acid isolation, making recombinant DNA, its transformation, selection, analysis, sequencing, hybridization and cytological methods. The student will acquire practical skills in the laboratory can isolate nucleic acids, separated them by electrophoresis on agar and polyacrylamide gels. Controls the method of PCR, microsatellite analysis, which can analyze and interpret the. The student is able to apply knowledge in laboratory practice and research.
Course contents:
Isolation, purification of DNA and cutting with restriction enzyme, making and transformation of recombinant DNA, technology of analysis and selection of recombinat DNA, DNA sequencing, creating and screening of DNA Library, making cDNA Library, preparation of genomic libraries, targeted gene expression, transgenic organisms, principles of genetic engineering of plants, animals and microorganisms.
Recommended or required reading:
Basic:
GÁLOVÁ, Z. -- BALÁŽOVÁ, Ž. -- MICHALÍK, I. -- LIBANTOVÁ, J. -- MORAVČÍKOVÁ, J. -- HRICOVÁ, A. -- MATUŠÍKOVÁ, I. Biotechnológie v rastlinnej produkcii. 1st ed. Nitra : Slovenská poľnohospodárska univerzita, 2008. 143 p. ISBN 978-80-552-0146-7.
TÓTH, D. -- BEŽO, M. -- BEŽOVÁ, K. -- BREZŇANOVÁ, I. -- BRINDZA, J. -- BULLA, J. -- FERENČÍK, I. -- ŠULAJOVÁ, L. -- GÁLOVÁ, Z. -- GODÁNY, A. -- HORECKÁ, T. -- HRAŠKA, Š. -- RAŽNÁ, K. -- CHRENEK, P. -- KAČÁNIOVÁ, M. -- KANTÍKOVÁ, M. -- KLUBICOVÁ, K. -- KMEŤ, V. -- SIEKEL, P. -- SOMMER, A. -- ŠAJBIDOR, J. -- ŠTEFANOVIČ, J. -- ŠTEFÚNOVÁ, V. Biologická bezpečnosť. Nitra : Slovenská poľnohospodárska univerzita, 2007. 463 p. ISBN 978-80-8069-846-1.
GÁLOVÁ, Z. -- BALÁŽOVÁ, Ž. -- CHRENEK, P. -- CHŇAPEK, M. -- LIBANTOVÁ, J. -- MATUŠÍKOVÁ, I. -- MORAVČÍKOVÁ, J. -- SALAJ, J. -- DRÁBEKOVÁ, J. Metódy a techniky génových manipulácií. 2nd ed. Nitra : Slovenská poľnohospodárska univerzita, 2018. 199 p. ISBN 978-80-552-1805-2.
GÁLOVÁ, Z. -- CHRENEK, P. -- BALÁŽOVÁ, Ž. -- MORAVČÍKOVÁ, J. -- CHŇAPEK, M. -- VIVODÍK, M. -- DRÁBEKOVÁ, J. Geneticky modifikované potraviny. 1st ed. Nitra : Slovenská poľnohospodárska univerzita, 2017. ISBN 978-80-552-1762-8.
GÁLOVÁ, Z. -- CHŇAPEK, M. -- BALÁŽOVÁ, Ž. -- VIVODÍK, M. -- MORAVČÍKOVÁ, J. Molekulárna biológia. 1st ed. Nitra : Slovenská poľnohospodárska univerzita, 2016. 134 p. ISBN 978-80-552-1550-1.
GÁLOVÁ, Z. -- BALÁŽOVÁ, Ž. -- LIBANTOVÁ, J. -- MATUŠÍKOVÁ, I. -- MORAVČÍKOVÁ, J. -- SALAJ, J. -- HRICOVÁ, A. Praktické cvičenia z molekulárnej biológie. 1st ed. Nitra : Slovenská poľnohospodárska univerzita, 2011. 67 p. ISBN 978-80-552-0657-8.

Recommended:
H. Lodishy a kol.: Molecular Cell Biology, USA, SAB Inc., 1995
J. Turňa a kol.: Rekombinantné DNA a biotechnológie, Alfa, Bratislava, 1989
J. Turňa a kol.: Techniky rekombinantných DNA. VEDA, 2004

Language of instruction: Slovak
Notes:
subject is taught only in the summer semester
Evaluation of course unit:
Assessed students in total: 205

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24,4 %15,6 %18,5 %17,6 %20,5 %3,4 %
Name of lecturer(s): doc. Mgr. Želmíra Balážová, PhD. (instructor)
prof. RNDr. Zdenka Gálová, CSc. (examiner, instructor, lecturer, person responsible for course)
Ing. Milan Chňapek, PhD. (instructor)
prof. Ing. Peter Chrenek, DrSc. (lecturer)
Last modification: 5. 3. 2019
Supervisor: prof. RNDr. Zdenka Gálová, CSc. and programme supervisor


Last modification made by Ing. Martin Mastík, PhD. on 03/05/2019.

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