Course syllabus PHD_P123_N - Callus Culture of Plants (FBFS - 2019/2020 - post-graduate studies)

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Slovak University of Agriculture in Nitra
Faculty of Biotechnology and Food Sciences
Course unit code:
Course unit title:
Callus Culture of Plants
Planned types, learning activities and teaching methods:
lecture1 hour weekly / 13 hours per semester of study (on-site method)

Agrobiotechnology - doctoral (elective), 1. year
Agrobiotechnology - doctoral (elective), 1. year
Level of study:
Prerequisites for registration:
Assesment methods :
During the semester there will be semester project and its presentation in 40 points and a final written exam in 60 points. To obtain A mark will be needed to obtain at least 93 points, for the evaluation of B mark at least 86 points, C mark at least 79 points, for the evaluation of D mark at least 72 points and for the evaluation of E mark at least 64 points.
Learning outcomes of the course unit:
The student will become familiar with methods of plant cell, tissue and organ cultures and their practical application in micropropagation and in setting up production plantings, in the production of synthetic seeds, in recovering propagating material from contamination, in cryopreservation (in vitro) plant material or application of in vitro cultures in other biotechnological techniques such as genetic modifications. The graduate will pin down the basic process of morphogenesis such as organogenesis and embryogenesis – zygotic, somatic or gametic. The graduate will obtain after the cours practical experiences in laboratory and they will learn essential principles of work with tissue cultures. They will be able to utilize all this knowledge not only in laboratory practice but also in research.
Course contents:
The course is excellent source to provide advanced knowledge of in vitro cultures condition, importance of plant growth regulators, organogenesis, somatic vs. zygotic embryogenesis, androgenesis, gynogenesis, understanding of different types of tissue cultures (meristem, nodal, anther and microspore cultures, callus and cell suspension cultures, protoplast cultures, embryocultures), about ways of germplasm protection, as well as importance of plant tissue cultures in green biotechnologies to students invoved in plant tissue culture research and plant biotechnology.
Recommended or required reading:
GÁLOVÁ, Z. -- BALÁŽOVÁ, Ž. -- MICHALÍK, I. -- LIBANTOVÁ, J. -- MORAVČÍKOVÁ, J. -- HRICOVÁ, A. -- MATUŠÍKOVÁ, I. Biotechnológie v rastlinnej produkcii. 1st ed. Nitra : Slovenská poľnohospodárska univerzita, 2008. 143 p. ISBN 978-80-552-0146-7.
RAŽNÁ, K. -- BEŽO, M. -- KUTIŠOVÁ, J. -- ŽIAROVSKÁ, J. -- GAJDOŠOVÁ, A. -- OSTROLUCKÁ, M G. -- HRICOVÁ, A. -- LIBIAKOVÁ, G. Explantátové kultúry rastlín. 1st ed. Nitra : Slovenská poľnohospodárska univerzita, 2009. 127 p. ISBN 978-80-552-0323-2.

George, E.F. a kol. Plant propagation by tissue culture. 3. vyd. Springer, 2008. 51s. ISBN 978-1-4020-5004-6 (Print) 978-1-4020-5005-3 (Online).

Language of instruction:
Evaluation of course unit:
Assessed students in total: 0

Name of lecturer(s):
prof. RNDr. Zdenka Gálová, CSc. (examiner, lecturer, person responsible for course)
Last modification:
14. 1. 2019
prof. RNDr. Zdenka Gálová, CSc. and programme supervisor

Last modification made by Ing. Martin Mastík, PhD. on 01/14/2019.

Type of output: