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Ing. Juraj Medo, PhD.
Identification number: 1180
University e-mail: juraj.medo [at] uniag.sk
 
lecturer CSc.PhD. - Department of Microbiology (FBFS)

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Basic information

Basic information about a final thesis

Type of thesis: Diploma thesis
Thesis title: Metagenomic analysis of microorganisms associated with maize plants
Written by (author): Ing. Nicole Bučeková
Department: Department of Microbiology (FBFS)
Thesis supervisor: Ing. Juraj Medo, PhD.
Opponent:Ing. Michal Gábor, PhD.
Final thesis progress:Final thesis was successfully defended.


Additional information

Additional information about the final thesis follows. Click on the language link to display the information in the desired language.

Language of final thesis:Slovak

Slovak        English

Title of the thesis: Metagenomic analysis of microorganisms associated with maize plants
Summary:Plant microbiome is one of the main factors in plant health and production. The manipulation and knowledge of microbiome has the potential to reduce the incidence of diseases, increase agricultural production, and reduce chemical processes resulting in more sustainable farming practices. This work is focused on the identification of maize microbiome, which is one of the most important cultivated crops worldwide. The composition of prokaryotic microorganisms that colonize plant tissues as well as soil environment has been identified and changes in microbial composition during the 3 plant growth phases (BBCH 15, 55 and 85) were also observed. The microbiome was analyzed in 6 different niches (in plant-free native soil, in rhizosphere zone, in rhizoplane, in plant roots, in stem, and leaves. Microbiome composition was analyzed by amplicon sequencing V4 region of 16S rRNA gene on Illumina MiSeq. There was a significant difference in microbiome composition between niches. The most significant difference was found in the proportion of microorganisms in soil and plant and soil compartments, with the Acidobacteria phylum predominantly in the soil sections. The Proteobacteria phylum was also very common. The Proteobacteria phylum was predominant in plant tissues and the second most abundant phylum was Actinobacteria phylum which had the highest percentage in the BBCH 85 stage. The diversity expressed by the value of Shannon index was significantly influenced by both niche and the growth stage. When comparing the niche, the greatest difference is between soil and plant compartments. Diversity was not different between soil environments. Plant parts showed the highest diversity in roots. When comparing growth stages, diversity is the same in the first two stage while the last stage of BBCH 85 had lower diversity. The observed presence of microorganisms associated with maize plants can be further explored at a more detailed level. These findings can be used for further use in the cultivation of maize, its biological treatment and disease prevention.
Key words:microbiome, Illumina , Zea mays, 16S rRNA

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