Persons at SUA
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Basic information about a final thesis
Type of thesis:
|Thesis title:||The efficacy of fungicides against Fusarium species|
Written by (author):
|Department of Plant Protection (FAaFR)|
|Opponent 1:||doc. Ing. Tibor Roháčik, CSc.|
doc. Ing. Gabriela Juhásová, PhD.
Final thesis progress:
Final thesis was successfully defended.
Additional informationAdditional information about the final thesis follows. Click on the language link to display the information in the desired language.
Language of final thesis:
|Title of the thesis:||The efficacy of fungicides against Fusarium species|
|Summary:||ABSTRACT This work presents the results of field experiments realized on locality Malý Šariš in the years 2009 - 2010 as well as the results of laboratory experiments. The aim of the work was to study the interactions of fungicides with natural population of species from the genus Fusarium, involving in to the Fusarium head blight (FHB) and grain infection and in vitro studies of resistance mechanism of Fusarium species. Effect of the fungicides on Fusarium head blight and their influence to changes in species spectrum in population of Fusarium pathogens were evaluated. The content of mycotoxins (DON, ZEA, T-2 toxin) was determined in grains and the values of effective concentrations on the level of 50 % inhibition of growth of mycelium were determined. FHB in 2009 occurred in lower intensity than in 2010. The main cause for different levels of infestation was due to different weather conditions during the years. In both years the dominant pathogens, associated with Fusarium head blight, was determined to be the Fusarium graminearum and Microdochium nivale. The same species prevalence was detected also in symptomatic samples of grain. The most effective preparations in 2009 were tebuconazole, tebuconazole + prothioconazole and prothioconazole + fluoxastrobin. In the year 2010 the best effect was observed also for the combination of prothioconazole + fluoxastrobin. Applied fungicides have an impact on changes in species spectrum of pathogens in the spikelet and also in grain. Preparations containing substances belonging to the chemical class of triazoles reduced the occurrence of species of Fusarium, while the combination of chemical groups strobilurin + triazole eliminate the incidence of Microdochium nivale species better. The content of mycotoxins in grain was below the values stated in legislation except the fraction of rose grain separated from infected heads in 2009. Such universal product, which would generally be able to decrease the amount of all three tested mycotoxins has not been found. Sensitivity/resistance was tested on five species of Fusarium (F. avenaceum, F. culmorum, F. graminearum, M. nivale and F. poae). The most sensitive species was M. nivale, while the least sensitive species were F. graminearum and F. avenaceum. Each species of Fusarium responded to the tested concentrations of active substances in different way. It has been shown a different effect of specific preparations against specific types of Fusarium species. In all cases reduced susceptibility to the registered dose (preparations with chlorothalonil as active ingredient) was noticed with exception of M. nivale species. This species showed decreased susceptibility to cyproconazole + propiconazole. Low resistance was manifested in all kinds of Fusarium species in different product: F. avenaceum (prothioconazole + fluoxastrobin), F. culmorum (prochloraz), F. graminearum (spiroxamine + tebuconazole + triadimenol), M. nivale (tebuconazole + prochloraz) and F. poae (metconazole).|
fungicides, EC 50, resistance, Fusarium head blight, mycotoxins, Fusarium
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