Persons at SUA
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Basic information about a final thesis
|Type of thesis:||Diploma thesis|
|Thesis title:||Detection of single nucleotide polymorphism (SNP) rs22114085 associated with canine atopic dermatitis using by PCR-RFLP method|
|Written by (author):|
|Department:||Department of Genetics and Animal Breeding Biology (FAaFR)|
|Thesis supervisor:||doc. Ing. Martina Miluchová, PhD.|
|Opponent:||Ing. Jana Hanusová, PhD.|
Final thesis progress:
|Final thesis was successfully defended.|
Additional informationAdditional information about the final thesis follows. Click on the language link to display the information in the desired language.
|Language of final thesis:||Slovak|
Title of the thesis:
|Detection of single nucleotide polymorphism (SNP) rs22114085 associated with canine atopic dermatitis using by PCR-RFLP method|
In the first part of this thesis, we have focused on providing theoretical information dealing with skin diseases in dogs, where we approached their diagnosis, the cause of and treatment with an emphasis on atopic dermatitis, the occurrence of which has an increasing trend. Atopic dermatitis is in part genetically determined disease, hence, our research focused on the practical part of finding an association between SNP rs22114085 and the disease. Genomic DNA was isolated from the saliva of dogs that have been estimated prevalence of the disease. To find reference SNP polymorphisms, we used PCR-RFLP method, in which we used the restriction enzyme DdeI, which digested PCR products obtained from the known sequence. The research we included samples from a total of 62 dogs, which were represented the most numerous breed Czech schnauzer with the number of 45 dogs. In the analyzed population of dogs were detected genotypes CC, CT, TT, where C allele was present in all dogs with atopic dermatitis with different frequencies of occurrence of 0.25 to 1 on the basis of which we can assume an association between the C allele and atopic dermatitis.
|atopic dermatitis, skin diseases, rs22114085, SNP, dog, PCR-RFLP|
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