Persons at SUA
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Basic information about a final thesis
|Type of thesis:|
DNA damage in male reproductive cells - mechanisms, causes and consequences
|Written by (author):||Bc. Barbara Terebesiová|
|Ing. Eva Tvrdá, PhD.|
Final thesis progress:
Final thesis was successfully defended.
Additional informationAdditional information about the final thesis follows. Click on the language link to display the information in the desired language.
|Language of final thesis:|
|Title of the thesis:||DNA damage in male reproductive cells - mechanisms, causes and consequences|
|Summary:||Oxidation and reduction reactions, which are an inseparable part of the metabolism of male reproduction cells affect the formation of free radicals. If the radicals exceed the antioxidant capabilities of the organism, oxidative stress uprises. This is one of the main causes of sperm DNA damage due to DNA fiber disruption and base modification. Apart from oxidative stress, damage of the deoxyribonucleic acid in male reproductive cells is also caused by various other factors, such as damage during spermatogenesis, abnormal spermatid maturation, age, clinical procedures and enviromental toxicity. Over the years methods, which serve for analysis of the DNA damage in sperm have been developed. DNA fragmentation may be repared not only in sperm, but also in the fertilized egg. Practical part of this bachelor thesis described the effect of trivalent iron ions on oxidative damage of DNA in bovine sperm. Samples were checked in time intervals of 0, 2, 8, 16 and 24 hours. The trivalent iron activated the oxidation damage in larger concentrations (>100 micromol.dm-3). Lower concentrations (<50 micromol.dm-3 ), did not have an inhibition on sperm DNA. During long-term cultivation (24 h), the recorded mean values of 8-Oxo-2'-deoxyguanosine (sample A: 31,02+-2,11 %) were higher than those of the 0 h cultivation (sample A: 10,19+-0,55 %). From these result a conclusion arises: the effect of the trivalent iron on oxidation damage of sperm DNA depends on the concentration of iron and the time, when measurements were taken: the lenght of the cultivation.|
|Key words:||oxidative stress, DNA damage, spermatozoa, iron|
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