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doc. Ing. Jana Maková, PhD.
Identification number: 1386
University e-mail: jana.makova [at]
associate professor CSc./PhD. - Department of Microbiology (FBFS)

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Basic information

Basic information about a final thesis

Type of thesis: Habilitation thesis
Thesis title:Microbial biomass and activity of microorganisms in agriculturally utilized soil
Written by (author): doc. Ing. Jana Maková, PhD.
Department: Faculty of Biotechnology and Food Sciences
Opponent 1:doc. RNDr. Dana Urminská, CSc.
Opponent 2:prof. Ing. Karel Voříšek, CSc.
Opponent 3:doc. Ing. Erika Gömöryová, CSc.
Final thesis progress:Final thesis was successfully defended.

Additional information

Additional information about the final thesis follows. Click on the language link to display the information in the desired language.

Language of final thesis:Slovak

Slovak        English

Title of the thesis:Microbial biomass and activity of microorganisms in agriculturally utilized soil
Summary:The aim of habilitation thesis was to study and characterize changes in microbial biomass and activity of microorganisms in agricultural soil use (arable soil, pasture). For quality indicators and soil health we examined microbial biomass carbon, microbial quotient, basal and potential respiration (with the addition of glucose) and substrate availability index. We also investigated the effect of selected chemical parameters (organic carbon, hot water extractable carbon, total nitrogen and soil reaction) on microorganisms and their activity in the soil. In arable soil the soil microbial biomass was not affected by soil type, but significantly higher value was determined in the pasture. Basal microbial respiration as opposed to potential respiration was affected by soil type and land use. Lower substrate availability, we found in pasture than arable soil, which was associated with a higher C/N ratio with an acid soil reaction and land use. In the second part we under field and laboratory conditions to assess the effect of fertilizer plant crop residues, manure, digestate, compost, bottom sediment and biological preparation Azoter on microbial biomass and microbial quotient. Activity of microorganisms was followed by measuring the enzymatic activity of microorganisms (dehydrogenase activity), activity of microorganisms in the processes of the nitrogen cycle (amount of biologically releasable nitrogen and nitrification), respiration of microorganisms and metabolic quotient. The influence of fertilization confirmed that the microbial biomass and most of us observed microbiological indicators reacted sensitively to changes in soil quality. Application of manure, digestate, compost and bottom sediment positive values increased microbial biomass and activity of microorganisms. Particular biological preparation Azoter improved with the application of digestate in less fertile soil biomass of microorganisms and increased respiration of microorganisms.
Key words:microbial biomass,, arable soil,, pasture,, respiration, , dehydrogenase activity

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